Yeast Extract for Friis Medium
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1910
1.Yeast (93.75 g) + Super Q Water (562.5 ml) per flask.
2.Place in incubator at 37°C for 20 minutes.
3.Heat to 90-100°C for 5 minutes.
4.Cool on ice.
5.Pour into centrifuge bottles and centrifuge at 2500 rpms for 30 minutes (4°C).
6.Filter supernatant through glass pads twice.
7.Measure into bottles and label.
8.Autoclave for 2-5 minutes at 115°C.
9.Freeze in 45 and 90 ml aliquots.
125 g yeast per 750 ml water = 1 batch
Yield is approximately 700 ml.
