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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 英文名:
SMM 293-TII Expression Medium (Serum free, complete medium)
- 规格:
1L
SMM 293-TII无血清培养基
产品描述:
| SMM 293-TII培养基是专门用于HEK293细胞悬浮培养和瞬时转染的新一代无血清培养基,细胞培养性能和瞬时转染后的重组蛋白产量均明显优于SMM 293-TI及其他同类产品。产品无血清,无抗生素,无动物源性组分,有利于建立稳定的HEK293细胞表达系统。在转染后的表达阶段同时配套使用加料液SMS 293-SUPI能够实现细胞的长时间培养,获得更高的蛋白产量。 |
产品特点:
| • HEK293细胞瞬时转染专用培养基 | • 即用型完全培养基,不用添加其他组分 |
| • 转染前后无需更换培养基 | • 无动物源组分、无血清、无抗生素 |
| • 适用于瞬时转染和稳定株转染以及培养 | • 适用于细胞的悬浮培养和大规模的反应器培养 |
| • 细胞生长密度及重组蛋白产量明显高于同类产品 |
产品参数:
| 外观 | 澄清红色溶液 | 适用细胞株 | HEK293EBNA及相关的细胞(如HEK293F、HEK293E、 HEK293H 、HEK293S、HEK293FT) |
| 渗透压 | 275±10 mOsm/kg | 细胞接种密度 | 0.3-0.4×106 cells/mL |
| pH值 | 6.9–7.4 | 倍增时间 | < 26 hrs(因细胞类型、状态影响而存在数值变化) |
| 无菌检测 | 无菌 | 细胞培养评价 | 优良 |
| 内毒素 | < 10 EU/mL | 产品用途 | 仅用于科学研究,不推荐用于人类或动物的诊断和治疗 |
| 有效期 | 6个月 | 储存条件 | 2-8 ℃,避光 |
SMM 293-TII培养基为澄清红色透明液体,2~8℃避光保存,使用过程中请勿反复预热。
产品使用效果:
图1. SMM 293-TII生长性能检测。相同培养条件下,检测SMM 293-TII与SMM 293-TI及知名进口品牌HEK293
细胞无血清培养基中生长的HEK293F细胞密度随培养时间的变化。
图2. SMM 293-TII蛋白表达量检测。使用相同的Fc标签重组表达质粒转染培养在SMM 293-TII和测其它品牌同
类无血清培养基中的HEK293F,在转染后不同时间收样检测细胞的蛋白产量。
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- 作者
- 内容
- 询问日期
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Generating stable cell lines in HEK293
Generating stable cell lines in HEK293 Prior to transfection, it is recommended that you linearize your pcDNA gene construct. Linearizing will decrease the likelihood of the vector integrating into the genome in a way
Construction and Characterization of Adenovirus Vectors
stock solution with H2 O and sterilize by autoclaving for 45 min at 121°C. Complete medium Dialysis buffer (10 mM Tris-HCl, pH 8.0) Sterilize by filtration through a 0.2-µm filter. DNase I (Ad) Ethidium bromide
Lonza 12-765Q Pro293S-CDM, for suspension cells, 1L Lonza 12-764Q Pro293A-CDM, for adherent cells, 1L Lonza BE02-030Q ProVero-1 NAO Medium for Vero Cells, 1L Lonza 12-749Q UltraMDCK Serum-free Medium, 1L 杂交瘤细胞培养基 Lonza BE02










