• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        The Voltage-Clamp Fluorometry Technique

        互联网

        561
        Ion channels are the cell's gatekeepers. These proteins selectively allow ionic current to flow down its electrochemical gradient. In some cases, specialized chemical or voltage sensing domains respond to environmental changes and signal the cell to adjust its internal chemistry in response to its surroundings. Because of their importance in cell function, channels have been the focus of intense study at the functional and structural level. Here we describe the optical technique voltage-clamp fluorometry (VCF) which is used to monitor the functional state and probe the structural rearrangements that take place as ion channels are activated by voltage. VCF combines electrophysiology, molecular biology, chemistry, and fluorescence into a single technique. Our focus is on voltage-gated ion channels, but the technique described can be applied to other proteins. We describe the cut open vaseline gap configuration (COVG) for VCF recording.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序