• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Automated Voltage-Clamp Technique

        互联网

        879
        The voltage-clamp electrophysiology method is the gold standard for measuring the function of ion channels. In the past, this technique has had limited applicability in pharmaceutical drug discovery because of its low throughput, steep learning curve, and challenges in standardization of the experiments. Recently, new electrophysiology platforms have been developed, which are based on the use of planar electrodes. One key advantage of the new electrode geometry is that it makes the process of cell-to-electrode sealing amenable to automation, thus increasing the throughput and significantly reducing the skill-set needed to run the experiments. The further addition of computer-controlled fluidics, voltage-clamping electronics, and automated data handling makes it possible to perform multiple electrophysiology experiments in parallel with a high degree of consistency and in completely automated mode. Among the new offerings for automated voltage clamp, one of the systems, PatchXpress/Sealchip, is quickly becoming the new gold standard for the quantification of ion channel function. In this chapter, we provide an overview of the new planar patch-clamping platforms and describe how electrophysiology experiments are performed on the PatchXpress/Sealchip automated system.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序