Multiplex Integrating Waveguide Sensor: Signalyte™-II
互联网
926
A platform to detect multiplex fluorescent labels was developed based on liquid phase implementation of the Integrating Waveguide
Sensor detection principles. The liquid sample is held in a capillary cuvette with a lens at one end. The excitation light
incident on the cuvette at 90� angle. The emitted fluores cence is efficiently gathered and propagated to the end of the waveguide
cuvette, exiting via the lens to the detector. The capillary cuvette acts as a waveguide to efficiently gather the emission
signal, providing high detection sensitivity for small sample sizes.
Excitation sources ranging from 470 to 635 nm are four high-powered LEDs, allowing for multiplex fluorescence assays and a
spectrometer is used to collect the signal from 390 to 790 nm. The cuvette can hold 1–35 μL samples. This technology can be
used for a wide variety of assays and detection needs, such as FRET, end point PCR reading, immunoassays, chemiluminescence
detection, multiplex quantum dots assays, polarization assays, etc.
