Beta-galactosidase Reporter Gene Assay (Liquid Form)
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<center> <p> <u><font color="#800000"><font>Beta-galactosidase Reporter Gene Assay (Liquid Form)</font> </font> </u></p> </center>
REFERENCE: Hoffman, G., Garrison, T. R., and Dohlman, H. G., Analysis of RGS proteins in Saccharomyces cerevisiae , Methods Enzymol. 344:617-631, 2002.
1. Grow a starter culture at 30 C shaking (250 rpm) until it reaches saturation.
3. Grow at 30 C shaking (250 rpm) until the OD600 nm ~ 0.8 (this is usually done overnight).
Updated 01/23/02
