Development of Simian Retroviral Vectors for Gene Delivery

The development of retroviral gene transfer vectors has provided an effective way to deliver foreign genes into mammalian cells. This chapter will focus on the development of a simian retrovirus (SRV) gene delivery system. This includes the identification of the minimal packaging signal of a type D serogroup 2 SRV D2/RHE/OR/V1, construction of the SRV transduction and packaging vectors, generation of the SRV specific packaging cell line and production of the replication-deficient recombinant SRV.