• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        【分享】QUMA:一种新的分析甲基化实验数据的方法

        丁香园论坛

        4556

        发表在
        Nucleic Acids Research, doi:10.1093/nar/gkn294
        Advance Access published online on May 16, 2008

        摘要:Bisulfite sequencing, a standard method for DNA methylation profile analysis, is widely used in basic and clinical studies. This method is limited, however, by the time-consuming data analysis processes required to obtain accurate DNA methylation profiles from the raw sequence output of the DNA sequencer, and by the fact that quality checking of the results can be influenced by a researcher's bias. We have developed an interactive and easy-to-use web-based tool, QUMA (quantification tool for methylation analysis), for the bisulfite sequencing analysis of CpG methylation. QUMA includes most of the data-processing functions necessary for the analysis of bisulfite sequences. It also provides a platform for consistent quality control of the analysis.

        文章还没有看完,我做的是BSP+直接测序法,最近一直在考虑数据的分析方法,对于甲基化+测序法的数据统计一直都有很大的疑问,不知道怎样统计才能得到客观并且逻辑严密的结果,在此抛砖引玉,欢迎广大做甲基化的战友讨论!

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序