【求助】解释Taqman-based microRNA assays

Taqman-based microRNA assays具体是什么实验方法？

是什么时候出的？

现在应该在哪里？

要求什么实验条件啊？

哪里可以做呢？

拜托各位大侠了

具体请参考下面这篇文章：Real-time quantification of microRNAs by stem–loop RT–PCR学习要自己慢慢看，什么都告诉你谁都可以做啊。

The basis of TaqMan® MicroRNA Assays is a target-specific stem-loop structure, reverse-transcription primer .Its innovative design overcomes a fund-amental problem in miRNA quantitation: the short length of mature miRNAs(~22 nt) prohibits conventional design of a random-primed RT step followed by a specific real-time assay. The stem-loop accomplishes two goals: 1) specificity for only the mature miRNA target, and 2) formation of a RT primer/mature miRNA-chimera, extending the 5’ end of the miRNA. The resulting longer RT amplicon presents a template amenable to standard real-time PCR, using TaqMan assays.

感谢！！！