• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Micropropagation of Penthorum chinenseThrough Axillary Buds

        互联网

        460
        Penthorum chinense Pursh is a traditional medicinal herb in China. Micropropagation protocol of this plant has been established. The shoot induction rate is high by culturing nodal explants on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) as compared with kinetin (Kn). After 6 wks, the highest shoot formation (5.2) is achieved in 59.2% nodal explants cultured on MS medium combined with 2.0 mg/L BA. After 4 wk of subculture on the fresh MS medium, the highest shoot multiplication rate 6.4 is accomplished. MS medium containing 1.0 mg/L BA is most suitable for shoot propagation. Individual well developed shoots (0.8-to 1.0-cm long) are excised and culture on the MS medium containing with 0.5 mg/L indole-3-acetic acid (IAA), for rooting. Three weeks later, 98.8% of shoots had rooted with an average of ten roots per shoot. Plantlets transferred to soil were successfully acclimatized. This protocol will facilitate the conservation and propagation of this important medicinal plant.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序