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        2%多聚甲醛固定剂配方二则

        互联网

        3612

        METHOD 1:

        Formaldehyde preservative 2% formaldehyde solution in protein-free
        phosphate-buffered saline (PBS).

        Prepare as follows:

        Add 2 g paraformaldehyde powder (e.g., Sigma, St. Louis, MO) to 100 ml
        of 1 X PBS. Heat to 70
        ° C (do not exceed this temperature) in a fume hood
        until the paraformaldehyde goes into solution (note that this happens
        quickly as soon as the suspension reaches 70
        ° C). Allow the solution to
        cool to room temperature. Adjust to pH 7.4 using 0.1 M NaOH or 0.1 M HCl,
        if needed. Filter and store at 4
        ° C protected from light.

        METHOD 2:

        Formaldehyde preservative 2% formaldehyde solution in protein-free

        PBS.

        Prepare as follows:

        10% formaldehydundefined    20 ml
        10 x PBS
               10 ml
        Distilled water
             70 ml

        Cat.#04018), depolymerized paraformaldehyde, EM grade, methanol-free solution.

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