PG试剂盒，用于样本中多聚半乳糖醛酸酶活性检测，PG Act

CheKine™ 多聚半乳糖醛酸酶（PG）活性检测试剂盒（微量法）

货号：KTB1333-E

产品类型：细胞代谢检测试剂盒

产品概述

CheKine™ Micro Polygalacturonase (PG) Activity Assay Kit 是一款基于比色法的高性能检测试剂盒，用于定量检测多种生物样本（如植物组织、细菌、真菌及液体样本）中的多聚半乳糖醛酸酶（PG）活性。本试剂盒经过广泛样本验证，操作流程简便高效，适用于细胞代谢与细胞壁降解等相关研究。

检测原理

多聚半乳糖醛酸酶（PG）可催化多聚半乳糖醛酸水解，生成具有还原性醛基的半乳糖醛酸。在碱性加热条件下，还原糖与DNS试剂反应形成红棕色化合物，在540 nm波长处具有特征吸收峰。通过测定该波长吸光度的变化，可准确计算出PG酶活性。

主要特点与优势

• 适用样本类型广泛，涵盖植物、真菌、细菌及液体样本；
• 操作流程清晰简便，有利于提高实验效率与重复性；
• 试剂盒性能稳定，重现性良好。

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Standard

规格与价格

规格	价格（人民币）	货期
48 T/48 S	1389	现货（次日发货）
96 T/96 S	2306	现货（次日发货）

保存与运输条件

建议保存于4℃避光环境，保质期为6个月。本产品采用冰袋（蓝冰）运输，以确保试剂稳定性。

注意事项

• 推荐使用新鲜样本。如不立即实验，样本可在-80℃保存数周。解冻过程应严格控制温度与时间，室温解冻须在4小时内完成；
• 实验前请确保所有试剂及设备均处于适宜温度，以保证检测准确性。

背景信息

多聚半乳糖醛酸酶（PG，EC 3.2.1.15）是一种关键的果胶酶，广泛分布于植物、真菌和细菌中。其在果实软化、花粉授粉、种子发育及植物病原体侵染过程中发挥重要作用，通过降解细胞壁中的多聚半乳糖醛酸促进病程发展。

警告：本品仅供科研使用，不适用于人类或临床诊断。使用前请查阅所有相关法规与许可协议，用户须自行承担使用本品可能涉及的专利或合规风险。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Polygalacturonase (PG) Activity Assay Kit

Catalog Number:KTB1333-E

Product Type:Cell Metabolism Assay Kit

Product Overview

The CheKine™ Micro Polygalacturonase (PG) Activity Assay Kit is a high-performance colorimetric assay kit designed for the quantitative determination of polygalacturonase (PG) activity in a variety of biological samples, such as plant tissues, bacteria, fungi, and liquid samples. This kit has been extensively validated with diverse samples and features a simple and efficient operation procedure, making it suitable for research related to cell metabolism and cell wall degradation.

Assay Principle

Polygalacturonase (PG) catalyzes the hydrolysis of polygalacturonic acid, generating galacturonic acid with reducing aldehyde groups. Under alkaline heating conditions, the reducing sugar reacts with DNS reagent to form a reddish-brown compound, which exhibits a characteristic absorption peak at 540 nm. By measuring the change in absorbance at this wavelength, PG enzyme activity can be accurately calculated.

Key Features and Advantages

• Wide range of applicable sample types, including plants, fungi, bacteria, and liquid samples;
• Clear and straightforward operation procedure, enhancing experimental efficiency and reproducibility;
• Stable kit performance with excellent reproducibility.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Standard

Specifications and Pricing

Specification	Price (RMB)	Lead Time
48 T/48 S	1389	In stock (shipped next day)
96 T/96 S	2306	In stock (shipped next day)

Storage and Shipping Conditions

It is recommended to store the kit at 4°C protected from light; the shelf life is 6 months. This product is shipped with ice packs (blue ice) to ensure reagent stability.

Precautions

• Fresh samples are recommended. If not used immediately, samples can be stored at -80°C for several weeks. The thawing process must be carefully controlled in terms of temperature and time; thawing at room temperature must be completed within 4 hours;
• Before the experiment, ensure all reagents and equipment are at appropriate temperatures to guarantee assay accuracy.

Background Information

Polygalacturonase (PG, EC 3.2.1.15) is a key pectinase widely distributed in plants, fungi, and bacteria. It plays important roles in fruit softening, pollen pollination, seed development, and plant pathogen infection by degrading polygalacturonic acid in the cell wall to facilitate disease progression.

Warning:This product is for research use only and is not intended for human or clinical diagnostic use. Please review all relevant regulations and license agreements before use. Users are responsible for any patent or compliance risks associated with the use of this product.

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please contact Yakrin Company.