• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Epitope Mapping by Chemical Fragmentation

        互联网

        620
        The use of antigen fragments generated by specific chemical cleavage is a relatively simple “library” approach for epitope mapping in which overlapping fragments are screened with antibody on Western blots. It is widely applicable insofar as it is not restricted to recombinant antigens only, but the amino-acid sequence of the antigen must be known. It cannot be used for highly assembled epitopes, which will be affected by the denaturing conditions of cleavage and SDS-PAGE, as well as by fragmentation itself. Chemicals that cleave proteins at uncommon amino acids are used to produce fragments; cyanogen bromide (CNBr) cleaves C-terminal to methionine residues (1 ), nitrothiocyanobenzoic acid (NTCB) at Cys (2 ), iodosobenzoic acid (IBA) at Trp (3 ), and formic acid between Asp-Pro bonds (4 ). The fragments on Western blots can often be recognized unequivocally using M r s predicted from the sequence.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序