- ￥1000 - 6000
- 义翘神州
- 109143-MM13
- 北京
- 2025年08月12日
- WB, ELISA, FCM, ICC/IF
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京义翘神州科技股份有限公司
- 库存：
99
- 靶点：
FLAG
- 级别：
免疫学试剂
- 目录编号：
109143-MM13
- 克隆性：
单克隆
- 抗原来源：
Monoclonal Mouse IgG2a Clone #13
- 保质期：
12个月
- 抗体英文名：
Anti-DYKDDDDK (Binds to FLAG® Tag epitope) Antibody
- 抗体名：
Anti-DYKDDDDK (Binds to FLAG® Tag epitope) Antibody
- 标记物：
Unconjugated
- 宿主：
小鼠
- 免疫原：
A synthetic peptide corresponding to the DYKDDDDK-tag sequence.
- 亚型：
IgG2a
- 形态：
液体
- 应用范围：
WB, ELISA, FCM, ICC/IF
- 保存条件：
-20℃ to -80℃
- 浓度：
WB：0.2-1 μg/mL
- 规格：
20 µg/100 µg/1 mg
| 规格： | 20 µg | 产品价格： | ￥1000.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥2000.0 |
| 规格： | 1 mg | 产品价格： | ￥6000.0 |
Anti-DYKDDDDK (Binds to FLAG® Tag epitope) Antibody, Mouse Monoclonal General Information
Product nameAnti-DYKDDDDK (Binds to FLAG® Tag epitope) Antibody, Mouse Monoclonal
Validated applicationsWB, ELISA, FCM, ICC/IF
SpecificityRecognize N-terminal, C-terminal or internal DYKDDDDK-tag in fusion proteins (Binds to FLAG® Tag epitope).
ImmunogenA synthetic peptide corresponding to the DYKDDDDK-tag sequence.
PreparationThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the synthetic peptide corresponding to the DYKDDDDK-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
SourceMonoclonal Mouse IgG2a Clone #13
PurificationProtein A
Formulation0.2 μm filtered solution in PBS
ConjugateUnconjugated
FormLiquid
ShippingThis antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
StorageThis antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-DYKDDDDK (Binds to FLAG® Tag epitope) Antibody, Mouse Monoclonal Validated Applications
Application Dilution
Western Blot 0.2-1 μg/ml
ELISA 0.5-1 μg/ml
FCM 0.5-2 μg/Test
ICC/IF 10-50 μg/ml
Please Note: Optimal concentrations/dilutions should be determined by the end user.
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Murine Monoclonal Antibody Development
Major advances in the analysis of biomolecular structure have emerged from the development of highly specific monoclonal antibodies (MAb) by K�hler and Milstein in 1975 (1 ). MAb are produced from hybridoma cell lines that secrete a single
Monoclonal Antibody Affinity Chromatography
. These criteria are often best fulfilled for protein targets by antibody ligands. Monoclonal antibodies are the ligands of choice for most purposes, especially when a protein of unknown properties is being characterized. Monoclonal antibodies fulfill
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