Lipid Affinity Beads: From Identifying New Lipid Binding Proteins to Assessing Their Binding Properties
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Lipid affinity beads can be used to identify novel proteins with lipid binding capacity or to determine binding prerequisites of known lipid-binding proteins. Here we describe several applications for which this tool can be used and which considerations have to be taken into account. In addition to a precise protocol, several suggestions are made for experimental setups to facilitate identification of in vivo lipid binding targets.
