• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Analysis of Chromosomes of the Larval CNS by FISH and BrdU Labeling

        互联网

        465
        The central nervous system (CNS) of the third instar larva is the most convenient tissue in which to examine both mitotic chromosomes and the diploid interphase nucleus. It contains a mixture of cell types at various degrees of differentiation. Here, methods for examining the diploid nuclei of the third instar larva are presented. One method employs BrdU incorporation to label nuclei during the S-phase. A second uses fluorescence in situ hybridization (FISH) to determine where certain sequences are located. Both can be used on either mitotic chromosomes or interphase nuclei. These two methods can be used by themselves or in combination. When used in combination, a researcher can identify which cells have recently undergone the S-phase and examine the specific positions of various chromosomal regions in the interphase nucleus.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序