Yeast as a Platform to Explore Polyglutamine Toxicity and Aggregation
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Protein misfolding is associated with many neurodegenerative diseases, including neurodegenerative diseases caused by polyglutamine
expansion proteins, such as Huntington’s disease. The model organism baker’s yeast (Saccharomyces cerevisiae
) has provided important general insights into the basic cellular mechanisms underlying protein misfolding. Furthermore, experiments
in yeast have identified cellular factors that modulate the toxicity and the aggregation associated with polyglutamine expansion
proteins. Notably, many features discovered in yeast have been proven to be highly relevant in other model organisms and in
human pathology. The experimental protocols depicted here serve to reliably determine polyglutamine toxicity and polyglutamine
aggregation in yeast.