Optical Filters for FISH

Fluorescence in situ hybridization (FISH) is strongly dependent on good hardware, i.e., the microscope and the filter sets used. Here an introduction to fluorescence, fluorescence spectra and fluorescent dyes, as well as filters and their characteristics, is given. Hints on how to choose the right filters are provided. Standard filter sets for FISH, CGH and multicolor FISH are presented. Filter handling, including mounting, is outlined. Finally, a troubleshooting section, including suggested solutions to specific problems that arise in connection with fluorescence filters and their use, is given. Such information is essential for fluorescence microscopy in general and for FISH specifically.