Immunoaffinity, Capillary Electrophoresis, and Statistics for Studying Intact Alpha 1-Acid Glycoprotein Isoforms as an Atherothrombosis Biomarker
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Variations in the amino acid sequence, glycosylation, and/or other posttranslational modifications in glycoproteins give rise
to different molecules of the glycoprotein called forms. Qualitative and/or quantitative alterations in these forms are related
to pathophysiological situations in the individuals. In this study, a methodology to analyze these differences in forms of
the alpha 1-acid glycoprotein (AGP) between healthy individuals and patients with two different vascular diseases is detailed.
The whole methodology includes a sample preparation method based on immunochromatography, a capillary electrophoresis method
for separation of AGP peaks (isoforms), and statistical methods (Linear Discriminant Analysis) for sample classification.
As a result, it is shown that the methodology proposed allows studying the role of AGP isoforms as potential vascular disease
biomarkers.
