YAC Library Storage and Transport
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The development of the yeast artificial chromosome (YAC) cloning system (1 ) and the construction of YAC libraries with large numbers of genome equivalents (2 –6 ) provided a major impetus to mammalian genome mapping. These technical advances enabled the mapping of megabase-sized chromosomal regions (7 ,8 ), culminating in the first complete clone maps of single mammalian chromosomes (9 ,10 ).
