Nematode Genomic DNA Isolation
互联网
<center> <h1> Nematode Genomic DNA Isolation</h1> </center>
Materials:
|
Rich Agarose Plates: |
50 mM |
NaCl |
|
|
|
0.75 % |
BactoPeptone |
|
|
|
5 ug/ml |
cholesteroundefined |
|
|
|
1 mM |
CaCl2 * |
|
|
|
1 mM |
MgSO4 * |
|
|
|
25 mM |
K-PO4 (pH 6.0~undefined |
|
|
|
1.5 % |
agarose (not agar) |
|
|
|
|
~undefined add after autoclaving |
|
|
|
|
|
|
|
Worm Lysis Solution: |
100 mM |
Tris (pH 8.5) |
|
|
|
100 mM |
NaCl |
|
|
|
50 mM |
EDTA |
|
|
|
1 % |
SDS |
|
|
|
1 % |
beta-mercaptoethanol |
|
|
|
100 ug/ml |
proteinase K |
|
|
|
|
|
|
|
Phenol / CHCl3 |
25:24:1 |
Phenol : CHCl3 : isoamyl alcohol |
|
|
CHCl3 |
24:1 |
CHCl3 : isoamyl alcohol |
|
The Nematode Caenorhabditis elegans . editor: W.B. Wood (1988).
