DNA Labeling In Vivo: Quantification of Epidermal Stem Cell Chromatin Content in Whole Mouse Hair Follicles Using Fiji Image Processing Software
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DNA labeling in vivo using nucleoside analogues is a current experimental approach to determine cell proliferation rates in
cell cultures and tissues. It has also been successfully used to localize adult stem cell niches through the identification
of nucleoside label-retaining cells (LRC) in long-term experiments. A major hindrance of this methodology relies on the selection
of adequate procedures to quantify the nucleoside analogue content from image data files. Here we propose a simple procedure
using Fiji image processing software to accurately calculate nucleoside analogue retaining chromatin/total chromatin (LRC/DAPI)
signal ratios in the well-known mouse hair follicle stem cell niche.
