• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Genetic Analysis of Drug Resistance by Fluorescence In Situ Hybridization

        互联网

        588
        Tumor progression is driven by the accumulation of genetic changes, which, in aggregate, confer the malignant phenotype (1 ). Thus, tumor development proceeds via clonal divergence with selection for cells with, for example, a proliferative advantage, metastatic potential, or drug resistance phenotype (2 5 ). Although Southern blotting and polymorphic microsatellite markers are invaluable in providing information about the genetic alterations that underlie the development of solid tumors, the spatial relationships between tumor cells are destroyed during tissue processing (6 8 ). This leads to a loss of information on genetic heterogeneity and small subpopulations and presents an averaging of the genetic changes. Conventional karyotyping would determine both numerical and structural chromosome anomalies, but is largely impractical for the study of solid tumors, owing to the necessity for cell culture to produce metaphase spreads. Fluorescence in situ hybridization (FISH) is a powerful method for the analysis of genetic change in solid tumors (7 ,9 12 ). In particular, it allows the visualization of the genetic makeup of individual cells within their histological context (7 ). In general, FISH uses chromosome and region specific probes to assess rapidly copy number and rearrangements of chromosomes and genes. The genetically abnormal cells are detected by their aberrant hybridization pattern in the interphase nuclei. Thus, this method of analysis is generally termed “interphase cytogenetics” (9 ,10 ,12 ).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序