• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Genetic Analysis of Drug Resistance by Reverse In Situ Hybridization

        互联网

        468
        Drug resistance is a major factor that limits the effectiveness of cancer chemotherapy, and there is considerable evidence to suggest a genetic basis for many drug-resistant phenotypes. A major drawback to many of the conventional approaches used to investigate drug-resistance mechanisms is that some prior information or guesswork on the changes that have occurred is required, thus necessitating separate reagents to screen each possible change. When analyzing genetic changes for example, screening is limited to the use of gene or region specific probes as in Southern blot or microsatellite analysis. Recently, the molecular cytogenetic techniques of reverse in situ hybridization (REVISH), and its more advanced relative, comparative genomic hybridization (CGH), have been developed for the rapid global detection and mapping of genetic imbalances in tumor genomes (1 7 ). In REVISH, genomic DNA from the tumor is used as a complex probe and hybridized to normal metaphase chromosomes (6 ). Genomic sequences amplified in the tumor are then detected as an increased intensity of signal at the normal chromosomal position from which the amplified sequences are derived (1 ,6 ,7 ). For more accurate analysis of both loss and gain of genetic material, CGH is required; however, CGH involves complex fluorescence-comparison techniques and expert knowledge of chromosome identification (2 ). However, both REVISH and CGH are ideal methods to detect genetic changes associated with the acquisition of drug resistance in tumors (6 ,8 ).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序