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        Blotting知识介绍

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        1172

        The transfer of biomolecules onto a membrane support

        1)Preparing a cell-free extract containing the biomolecule(s) of interest
        2)Resolve the mixture by gel electrophoresis
        3)Transfer the gel onto a membrane support
        4)Incubate the membrane with a detection system that specifically binds to the molecule of interest

        Southerns - Detection of DNA fragments on a blot
        Northerns - Detection of RNA fragments on a blot
        Westerns - Detection of proteins on a blot
        South-Westerns - Detection of DNA bound to proteins on a blot

        Blotting知识介绍

        Blotting知识介绍

        Blotting知识介绍

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