• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTPS Binding

        互联网

        676
        Localization of receptors in brain sections using autoradtographlc detection of radioligand binding has been an important technique in the neuroanatomical identification of a large number of neurotransmitter receptors. However, receptor autoradiography provides little information regarding the functional relevance of these sites and, in fact, does not establish which of the labeled receptor sites are actually coupled to intracellular signaling mechanisms. Fortunately, with the family of G protein-coupled receptors (GPCR), signal transduction is mediated at the level of the transducer itself (i.e., at the point at which receptors activate the α-subunits of G proteins to bind guanosine 5′-triphosphate [GTP]). The development of an assay for agonist-stimulated [35 S]guanylyl-5′-O-(γ-thio)-triphosphate ([35 S]GTPγS) binding, originally developed for receptors in isolated membranes, has provided an excellent opportunity to apply this process to brain sections, thus allowing the visualization of receptor-activated G proteins in specific brain regions.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序