Measurement of Dopamine Uptake in Neuronal Cells and Tissues

Biogenic amines such as dopamine (DA), norepinephrine, and serotonin (5-hydroxytryptamine) are key neurotransmitters found in the central nervous system (CNS). Major DA containing cells, nuclei, and neurons are located in retina, olfactory bulb, hypothalamic-pituitary, nigrostriatal, mesolimbic, and mesocortical areas. Stimulation of DA containing neurons releases DA from storage vesicles at the nerve terminals into the synaptic region. Binding and activation of the high-affinity postsynaptic receptors by DA or DA agonists within the tissue will manifest changes in physiological and behavioral responses. Central DA receptors are known target sites for typical antipsychotic drugs as well as dopaminomimetic anti-Parkinson’s drugs. Therefore, the amount and duration that DA accumulates at the synaptic region will determine the intensity of DA receptor responses.