High-Throughput Functional Detection of NMDA Receptor Activity

A prominent current paradigm in the discovery of potential drugs directed toward therapeutic targets relies on the combination of brute force and specificity. The increasing number of entities in pharmaceutical company compound libraries, frequently surpassing 10⁶ , dictates the use of a high-throughput screening (HTS) process. In practice, large numbers of individual compounds are quickly tested for their ability to interact with, and affect a change in, a previously identified and validated target protein. The heterologous expression of cloned receptor proteins in defined cell lines provides the specificity of the interaction.