• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Analysis of Gene Expression in Xenopus Embryos

        互联网

        524
        Determining the expression pattern of a gene of interest is critical to understanding when, where, and how it may function during development. This chapter describes methods for determining the localization and expression levels for both mRNA and protein. Some of these methods can be described as quantitative or semi-quantitative while others are considered more qualitative in nature. To determine the spatial localization of mRNA expression, RNA in situ hybridization can be used on both whole-mount or sectioned embryos. Northern blot and qPCR are more quantitative methods for analyzing mRNA expression levels. For determining protein localization, antibody staining either by immunocytochem-istry or immunofluorescence can be performed on both whole-mount or sectioned embryos; whereas Western blot is the method generally used for quantifying protein expression levels.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序