Seed sterilization for cereals
互联网
1) wash 10' with tap H2O
2) wash w/ 4xV w/ 0.2% AgNO3
3) wash 2x w/ sterile H2O
4) wash 1x w/ 100mM NaCl
5) wash 3x w/ sterile H2O
6) plate w/ 100ug/ml chloramphenicol
100U/ml Nystatin
OR
1) wash 1' 96% EtOH, aspirate
2) wash 10' 30% commercial bleach w/ 1ul/ml 20% TritonX100
3) wash 5x w/ sterile H2O
4) plate as above
上一篇:叶绿体色素的提取、分离和理化性质 下一篇:Progeny analysis of plants


![///蛋白Recombinant Glycine max 2S seed storage albumin protein重组蛋白2S albumin; GM2S-1; Napin-type 2S albumin 3) [Cleaved into: 2S albumin small chain; Aspartic acid-rich peptide; Lunasin); 2S albumin large chain; 8 kDa methionine-rich protein; 8 kDa MRP)]蛋白](https://img1.dxycdn.com/p/s14/2024/0914/429/2534610676379384381.jpg!wh200)






