Seed sterilization for cereals

互联网2013-12-30

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1) wash 10' with tap H2O

2) wash w/ 4xV w/ 0.2% AgNO3

3) wash 2x w/ sterile H2O

4) wash 1x w/ 100mM NaCl

5) wash 3x w/ sterile H2O

6) plate w/ 100ug/ml chloramphenicol

100U/ml Nystatin

1) wash 1' 96% EtOH, aspirate

2) wash 10' 30% commercial bleach w/ 1ul/ml 20% TritonX100

3) wash 5x w/ sterile H2O

4) plate as above

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