Seed sterilization for cereals
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1) wash 10' with tap H2O
2) wash w/ 4xV w/ 0.2% AgNO3
3) wash 2x w/ sterile H2O
4) wash 1x w/ 100mM NaCl
5) wash 3x w/ sterile H2O
6) plate w/ 100ug/ml chloramphenicol
100U/ml Nystatin
OR
1) wash 1' 96% EtOH, aspirate
2) wash 10' 30% commercial bleach w/ 1ul/ml 20% TritonX100
3) wash 5x w/ sterile H2O
4) plate as above
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