Fixation and Staining Parameters
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<center> <b><font>Fixation and Staining Parameters</font> </b></center>
|
Antigen |
Fixation |
Fixation time |
<center> <b>Antibody</b></center> |
<center> <b>Dilution</b></center> |
<center> <b>Ref</b></center> |
|
microtubules |
100% MeOH |
5 min @ RT |
<center> DM1a</center> |
<center> 1:500</center> |
|
|
actin |
4% Formaldehydundefined |
8 min @ RT |
<center> C4</center> |
<center> 1:500</center> |
<center> 1</center> |
|
POD-1
|
Formaldehyde or MeOundefined |
8 min @ RT |
<center> 11-1</center>
<center> 11-9</center> |
<center> 1:1000</center>
<center> 1:1000</center> |
<center> 1</center> |
|
PAR-1 |
100% MeOH |
15 min @ -20 deg |
<center> PAR-1</center> |
<center> 1:5</center> |
<center> 2</center> |
|
PAR-3 |
100% MeOH |
15 min @ RT |
<center> PAR-3</center> |
<center> 1:20</center> |
<center> 3</center> |
|
P-granules |
100% MeOundefined~K~Hp~M~2~1~0~0~0~K~Htd~M~2~1~0~0~0~Ktd~M~2~1~0~0~0~0~Kp~M~2~1~0~0~0~0~010_min_@_4_deg~K~Hp~M~2~1~0~0~0~K~Htd~M~2~1~0~0~0~Ktd~M~2~1~0~0~0~0~Kp~M~2~1~0~0~0~0~0 <center> K76</center> |
<center> 1:1 or 1:2</center> |
<center> 4</center> |
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