• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        FACS Analysis for TRUS patients

        互联网

        4496

        Specimen collection:

        Whole blood is collected in Vacutainer CPT (Becton-Dickinson) or 9 ml EDTA vials.

        Isolation of peripheral mononuclear cells:

        If CPT Vacutainer are used, we follow in principle the VACUTAINER CPT datasheet. Therefore the single steps are only described very shortly. For more information please always have a look into the datasheet!! Changes are given in cursive letters.

        Wash cell pellet a second time with PBS/2% horse serum and remove the supernatant with a Pasteur pipette and a waterpump as completely as possible.

        Specimen Collection with VACUTAINER CPT

        1. VACUTAINER CPT Tube with Sodium Citrate should be at room temperature.
          2. Collect blood in the tube using the standard technique for VACUTAINER Brand Blood Collection Tubes and mix blood with sodium citrate solution immediately after collection.
          3. Store tube upright at room temperature until centrifugation. Blood samples should be centrifuged within two hours of blood collection for best results. Remix the blood sample immediately prior to centrifugation by gently inverting the tube 8 to 10 times.
          4. Centrifugation: room temperature / 8-10 min at 1500 x g (=3000 rpm, swingout rotor in the Sorvall table centrifuge, RIA Lab, program 4).
          5. Collect the whitish cell layer under the plasma layer (monocytes/macrophages and platelets) with a (2 ml plastic) Pasteur pipette. Transfer suspension to a 50 ml size conical centrifuge tube, fill up with cold PBS PBS containing 2% (horse) serum (or 1% BSA)) to 30 ml. Immediately collect the cells by centrifugation for 7 min at 244 x g (=1100 rpm, swingout rotor in the Sorvall table centrifuge, RIA Lab, program 4). Remove supernatant by decanting to remove platelets.
          6. Wash cell pellet a second time with cold PBS / 2% horse serum and remove the supernatant with a Pasteur pipette and a waterpump as completely as possible. Suspend the cells in PBS.

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序