26The Production of Chemokine Specific Monoclonal Antibodies: Chemokine RANTES

Monoclonal antibodies (MAbs) produced through the technique of somatic cell fusion are fundamental tools in the exploration of chemokine biology. This chapter details the procedures that were used to generate a panel of MAbs directed against the human chemokine RANTES (1 ,2 ). The general approach described here should be broadly applicable to the generation of MAbs directed against other members of this cytokine family.