• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Hydrodynamic Delivery of DNA

        互联网

        944
        The study of gene expression and regulation relies on the introduction of DNA into cells, which can be accomplished by a procedure called transfection. In vitro studies with transfected cells expressing the introduced genes (trans-genes) have provided many valuable insights into gene function. However, in vitro transfection methods cannot provide the complete range of responses possible in intact organs and tissues, which have unique anatomy and consist of different cell types. Thus, in vivo studies in whole animals remain a critical element in gene-function studies. The effective and efficient in vivo delivery of DNA into whole animals has presented significant challenges. Although much work continues to be carried out to improve the more commonly used viral and nonviral vectors for gene delivery, recent progress in the application of naked DNA methods (1 -4 , and the chapters in this volume) has offered valuable alternatives. The obvious utility of direct introduction of DNA into tissues without the requirement for a viral vector has stimulated development of several alternative delivery methods. Of these, the hydrodyamics method (5 -8 ) offers a simple, convenient means for the expression of significant levels of transgenes in the organs, especially the liver, of mice.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序