Conformation-Sensitive Gel Electrophoresis

Conformation-sensitive gel electrophoresis (CSGE) was initially developed by Ganguly et al. in 1993 as a screening method to minimize the amount of nucleotide sequencing required when investigating large multiexon genes for mutations (1 ). It is based on the ability to distinguish between homoduplexed and heteroduplexed DNA fragments by electrophoresis under partially denaturing conditions.