Antibiotic concentrations for E. coli selection
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Antibiotic Concentrations
The following list shows recommended antibiotic concentrations for LB media or agar plates.
| Antibiotic | Concentration |
| Ampicillin | 100 μg/mL |
| Bleocin | 5 μg/mL |
| Carbenicillin | 100 μg/mL |
| Chloramphenicol | 25 μg/mL |
| Coumermycin | 25 μg/mL |
| Gentamycin | 10 μg/mL |
| Kanamycin | 50 μg/mL |
| Spectinomycin | 50 μg/mL |
| Tetracycline | 10 μg/mL |
Example: To make 100 mL of LB/ampicillin growth media, add 100 μL of a 100 mg/mL ampicillin stock (1000X stock) to 100 mL of LB.
Reagent List: Antibiotics
Unless otherwise indicated, the antibiotic powder can be dissolved in H2 0. Addgene recommends making 1000X stock solutions and storing aliquots at -20o C.
| Reagent | Catalog Number |
| Ampicillin | EMD Chemicals #171254 |
| Bleocin | EMD Chemicals #203408 |
| Carbenicillin | EMD Chemicals #205805 |
| Chloramphenicol | EMD Chemicals #220551 (dissolve with EtOH) |
| Coumermycin | Sigma #C9270 (dissolve with DMSO) |
| Gentamycin | EMD Chemicals #345814 |
| Kanamycin | EMD Chemicals # 420311 |
| Spectinomycin | EMD Chemicals # 567570 |
| Tetracycline | EMD Chemicals # 583411 |
Please note that the catalog numbers given in the list above are only examples, and there are many additional companies that supply these reagents.
