• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Detection of Chromosome Ends by Telomere FISH

        互联网

        495
        Vertebrate chromosomes end in a variable number of T2 AG3 repeats (1 ), which, jointly with associated proteins, are essential for chromosomal stability (for reviews, see refs. (2 4 ). Telomeres have important roles in essential cellular processes like replication, malignant transformation, and cellular aging (see ref. (5 8 ). Telomere repeats make up to 2–50 kb of DNA (1 ,9 ,10 ), which are complexed with TTAGGG repeat binding factor (TRF) proteins (11 ,12 ). At interphase, telomeres are dispersed throughout the nuclear lumen (13 ,14 ) and appear to be associated with the nuclear matrix (15 ). In most somatic cell types replicative shortening leads to an interchro-mosomal variation of terminal T2 AG3 repeats (14 ,16 ,17 ) which can be halted or restored by the DNA-dependent (RNP) polymerase telomerase (8 ,18 ). Furthermore, telomeres are key players in the chromosome-pairing process during meiosis. At the onset of mei-otic prophase the scattered premeiotic (somatic) telomere distribution is altered such that telomeres attach to the inner nuclear membrane and then move along it to cluster in a limited nuclear envelope sector at the onset of zygotene (see ref. 19 ). This bouquet formation is thought to contribute to homolog recognition and pairing (e.g., 19 -21 ).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序