Gene Expression Profiling to Characterize Anticancer Drug Sensitivity

This chapter presents a protocol for using cDNA microarrays to acquire gene expression pro files that characterize anticancer drug sensitivity. The protocol includes steps for drug exposure, RNA isolation, preparation of fluorescently labeled samples, microarray hybridization, data pro cessing, and data analysis. In addition to the detailed protocol, important experimental design issues are discussed, and some preliminary experiments are recommended.