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        Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer

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        Three fundamental technologies have emerged in genetic analysis that have widespread and immediate benefits. DNA synthesis, fluorescence-based DNA analysis, and the polymerase chain reaction (PCR) are being integrated for a range of applications, from forensics to the understanding and treatment of genetic disease. By combining the use of PCR and DNA sequencing, we now have a clear understanding of the molecular basis for many disease states (1 ). The technologies used to map and sequence the human genome are also being used in leading research establishments to detect DNA mutations leading to genetic diseases such as muscular dystrophy, cystic fibrosis, HIV, and cancer susceptibility (1 3 ). The detection of mutations within many disease-related genes can be automated using fluorescent labeled fragments generated by PCR or cycle sequencing. One of Applied Biosystem’s main goals is to develop genetic analysis technologies to meet the needs of the clinical and research settings of the future.
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