Visualization of mRNA Expression in the Zebrafish Embryo
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Examination of spatial and temporal gene expression pattern is a key step towards understanding gene function. Therefore, in situ hybridization of mRNA is one of the most powerful and widely used �techniques in biology. Recent advances allow the reliable and simultaneous detection of mRNA transcripts, or combinations of mRNA and protein, in zebrafish embryos.
Here we describe a standard protocol for visualizing the precise expression pattern of a single transcript or multiple gene products. The procedure employs fixation and permeabilization of embryos, �followed by hybridization with tagged antisense riboprobes. Excess probes are then washed and hybrids are detected by enzyme-mediated immunohistochemistry utilizing either chromogenic or fluorescent substrates.
![Recombinant-Arabidopsis-thaliana-NADH-dehydrogenase-ubiquinone-1-alpha-subcomplex-subunit-13-AMEE4NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A
Alternative name(s):
Protein MATERNAL EFFECT EMBRYO ARREST 4](https://img1.dxycdn.com/p/s14/2024/1230/578/8691945264010663781.jpg!wh200)
