噬菌体的生长
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1602
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Preparing Lawn Cells for M13 Cloning ( Life Technologies)
Lawn cells require the F' episome for M13 infection and may be prepared
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Streaking Lambda Phages (Donis Keller Lab)
Phage are streaked onto a medium to obtain an independent isolate prior to preparing a new lysate
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Phage Propagation (hancock Lab) (Accessible only by Internet Explorer)
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Helper Phage Preparation (Vesicle Trafficking)
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Picking Single Phage Plaques (Donis Keller Lab)
For isolating single bacteriophage lambda plaques to be used for making lysates
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Lambda plaque screening (Molecular Genetics Network Lab)
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Liquid Phage Lysates (Donis Keller Lab)
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Preparation of Phage Lysates (Goldberg Lab)
Very nice and detailed protocol including recipes for media preparation
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Gel Assay to Determine DNA Content of Phage Lysates (Donis Keller Lab)
A crude lysate gel assay can be performed to roughly quantitate the DNA in lysates. This is often a valuable time saving step to determine if the phage yield is sufficient to warrant continuing the procedure
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Titering Lambda Phages (Donis Keller Lab)
This procedure is used to find the number of viable phage particles in a lysate. The phage lysate is serially diluted and ...
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Phage Plate Stock Lysates (Donis Keller Lab)
To prepare phage lysates to be used for small or large scale phage DNA preps. This method usually produces lysates with titers of 2-8x10e10 pfu/ml.
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Long-Term Lambda Phage Storage (Donis Keller Lab)
Store phage in 7% DMS0.
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Phage and Cell Storage (Gimila Lab)
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Transfer of Phage DNA from Plaques to Nylon Membrane or Nitrocellulose (Donis Keller Lab)
To transfer phage DNA to nylon membranes so that a phage library can be screened by hybridization
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Assay for Phage Containing the ß- Galactosidase Gene (Donis Keller Lab)
This assay is used when working with phage vectors carrying the beta-galactosidase gene (often used for immunological screening). If the cloning event disrupts a normally functional copy of the gene in the vector the resulting plaques would appear clear in the assay. If the phages contain a functional beta-galactosidase gene they will form blue rings around their plaques.
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Plaque Lifts (Gimila's Lab)
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Identifying Plaques by Neutral Red Staining (LTI)
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Phage Titre and Solution Preparation (Gimila's Lab)
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Colony/Plaque Blotting (Gimila's Lab)
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Calculating Phage Titers (Goldberg Lab)
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Phage Media Diagram (Goldberg Lab)
procedures for phage media preparation
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Growth and Purification of 25-100 µg Lambda Clone DNA (Donis Keller Lab)
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Lambda Methods Media and Solutions (Donis Keller Lab)
- Isolation of Phage Resistant Mutants (Hancock Lab) (Accessible only by Internet Explorer)
