• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Isolation of Fungal Infection Structures from Plant Tissue by Flow Cytometry for Cell-Specific Transcriptome Analysis

        互联网

        476
        Many plant pathogenic fungi differentiate a series of highly specialized infection structures to invade and colonize host tissues. Especially at early stages of infection, the ratio of fungal to plant biomass is very low. To investigate cell-specific patterns of gene expression, it is necessary to purify the fungal structures of interest from infected plants. We describe here a method to isolate the biotrophic hyphae of Colletotrichum higginsianum from Arabidopsis leaves, based on a combination of pre-enrichment by isopycnic centrifugation followed by further purification by fluorescence-activated cell sorting. This protocol efficiently eliminates contamination by plant components and nontarget fungal cell-types. Moreover, the isolated cells remain alive, providing high-quality RNA for library construction. The method can be readily adapted for cell-specific transcriptome analysis in other plant–microbe interactions.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序