• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        The Identification of Signaling Molecules by the Yeast Two-Hybrid System

        互联网

        426
        The yeast two-hybrid system is a sensitive method to detect intracellular interactions between proteins. It has been successfully employed in the identification of novel interactors of several proteins, including adhesion molecules (for a general review on the method, see refs. 1 and 2 ). In general, the high sensitivity of the yeast two-hybrid system allows the detection of weak and transient interactions that escape biochemical analysis. Howewer, in some limited cases, interactions found with biochemical methods have not been reproduced in the yeast assay, possibly because of the lack of some posttranslational modifications. More generally, the high sensitivity of the yeast interaction assay may lead to the disclosure of false-positive protein-protein interactions. For this reason, it is always necessary to confirm the yeast interaction data using other methods of study for protein binding.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序