DNA mobility in gels
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1. Migration of marker dyes in native polyacrylamide non-denaturing gels
Gel % Bromophenol blue (BP) Xylene cyanole (XC) 3.5 100 460 5.0 65 260 8.0 45 160 12.0 20 70 20.0 12 45
2. Migration of marker dyes in polyacrylamide denaturing gels
Gel % Bromophenol blue (BP) Xylene cyanole (XC) 5.0 35 130 6.0 26 106 8.0 19 75 10.0 12 55 20.0 8 28
3. Relative positions of different DNA forms on Tris-acetate agarose gels
The exact distance between bands is influenced by percentage of agarose, time of electrophoresis, concentration of Ethidium bromide, degree of supercoiling and the size and complexity of the DNA.
