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        Quantification of Xenoprotein Electroinsertion in Mammalian Cells

        互联网

        529
        Recent studies reported the so-called “electroinsertion” of proteins having a membrane-spanning sequence into mouse red blood cell or Chinese hamster ovary cell membrane by exposing the cell suspension to electrical field pulses (1 6 ). The viability of the pulsed cells is not affected. New receptors are then present on the cell surface. It is proposed to use such a technology in clinical applications such as AIDS therapy (7 ). Genetically engineered CD4s are inserted in red blood cells (RBC) and act as lures to trap the virus. A critical parameter for the practical use of such a methodology is to know the number of inserted xenoproteins.
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