Using Total Internal Reflection Fluorescence Microscopy (TIRFM) to Visualise Insulin Action
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Total Internal Reflection Fluorescence Microscopy is a powerful application of fluorescence microscopy that allows selective imaging of fluorescent molecules that are either in or close to the plasma membrane of a cell. Thus, it is ideally suited to imaging the trafficking of molecules to and from the plasma membrane. Here we describe its use to study the trafficking and exocytosis of the glucose transporter (GLUT4) in response to insulin stimulation.
