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        Expression of NMDA Receptor Channel Subunit Proteins Using Baculovirus and Herpesvirus Vectors

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        Foreign gene transfer and expression in the cells are among the most important techniques for determining the characteristics and function of cloned genes. Glutamate receptor genes have been expressed by RNA injection of Xenopus oocytes or transfection of mammalian cells, such as HEK, CHO, and COS cells, and bacterial Escherichia coli cells. Viral vectors, such as baculovirus (Autographa californica nuclear polyhedrosis virus, AcNPV) or herpesvirus (herpes simplex virus-1, HSV-1), have been also used because of high efficiency of gene transfer. Existing viral clones expressing recombinant glutamate receptor proteins (1 25 ) are summarized in Table 1 .
        Table 1  Summary of Viral Vectors Expressing Glutamate Receptor Proteins

        Viral vector, mouseGluR/ratGluR

        Reference

        Baculovirus vector

         

        α1

        1–5

        α2/GluRB

        3,6–9

        (α4)/GluRD

        9–11

        (β2)/GluR6

        12–14

        ζ1/NMDAR1

        15–17

        ε1

        18 and this study

        ε2

        18 and this study

        δ2

        18 and this study

        mGluR1 (rat)

        13

        Herpesvirus vector

         

        (α1)/GluR1

        19

        (β2)/GluR6

        20–22

        ζ1

        This study

        Adenovirus vector

         

        (a1)/GluR1

        23

        (a2)/GluR2

        23

        (ζ1)/NMDAR1 (antisense)

        24

        Adenoassociated virus vector

         

        (ζ1)/NMDAR1 (antisense)

        25

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