Expression of NMDA Receptor Channel Subunit Proteins Using Baculovirus and Herpesvirus Vectors
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Foreign gene transfer and expression in the cells are among the most important techniques for determining the characteristics and function of cloned genes. Glutamate receptor genes have been expressed by RNA injection of Xenopus oocytes or transfection of mammalian cells, such as HEK, CHO, and COS cells, and bacterial Escherichia coli cells. Viral vectors, such as baculovirus (Autographa californica nuclear polyhedrosis virus, AcNPV) or herpesvirus (herpes simplex virus-1, HSV-1), have been also used because of high efficiency of gene transfer. Existing viral clones expressing recombinant glutamate receptor proteins (1 –25 ) are summarized in Table 1 .
Table 1 Summary of Viral Vectors Expressing Glutamate Receptor Proteins
|
Viral vector, mouseGluR/ratGluR |
Reference |
|---|---|
|
Baculovirus vector |
|
|
α1 |
1–5 |
|
α2/GluRB |
3,6–9 |
|
(α4)/GluRD |
9–11 |
|
(β2)/GluR6 |
12–14 |
|
ζ1/NMDAR1 |
15–17 |
|
ε1 |
18 and this study |
|
ε2 |
18 and this study |
|
δ2 |
18 and this study |
|
mGluR1 (rat) |
13 |
|
Herpesvirus vector |
|
|
(α1)/GluR1 |
19 |
|
(β2)/GluR6 |
20–22 |
|
ζ1 |
This study |
|
Adenovirus vector |
|
|
(a1)/GluR1 |
23 |
|
(a2)/GluR2 |
23 |
|
(ζ1)/NMDAR1 (antisense) |
24 |
|
Adenoassociated virus vector |
|
|
(ζ1)/NMDAR1 (antisense) |
25 |
