Analysis of Migration in Primary Ureteric Bud Epithelial Cells

Kidney development has been widely used as a model system to study molecular control of inductive tissue interactions and mechanisms through which branching organs form. Due to lacking or poor methods, less focus has been in understanding details of cellular events that accomplish example ureteric bud (UB) branching. In order to form a branch point, cells need to proliferate, move in relation to each other, and change their shape as well as adhesive properties. In this chapter, detailed description is given how to set up primary UB epithelial cell cultures and study cell motility in these cells.