Determination of Fc-Mediated Antibody-Effector Functions by Chromium Release Assay

Monoclonal antibodies (mAbs) can exert their biological functions by a large variety of mechanisms and have therefore become a major part in the treatment of diseases like viral infections and cancer. For the clinical efficacy of many mAbs Fc-mediated effector functions like antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) play an essential role. This protocol describes a simple method to test whether a monoclonal antibody is able to induce ADCC or CDC using a chromium release assay.