Preparation of Blotted Membrane for Protein Microsequencing

This chapter aims to provide a step-by-step procedure in preparing protein samples of limited quantities for partial sequence analysis. Its content will cover a brief introduction on various types of sequenators thus far developed, with the emphasis on the latest technology of gas-liquid-phase sequenator. Detailed descriptions will be focused on how to electroblot protein samples onto polyvinylidene fluoride (PVDF) membrane. The method enables the beginner to prepare protein blots properly for direct microsequencing using currently available automated gas-liquid-phase sequenators.